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The overall number of 1536 complex points was collected in the acquisition dimension and 5000 hypercomplex points were distributed over the indirectly detected dimensions.
The overall number of 1536 points was collected in the acquisition dimension and 2500 hypercomplex points were sparsely distributed over the indirectly detected dimensions.
In all, 1536 complex points were acquired in the direct dimension and the overall number of 5000 hypercomplex points was non-uniformly distributed over the indirectly detected dimensions.
The on-grid Poisson disc sampling36 was utilized in the present application to distribute individual acquisition points in the indirectly detected dimensions.
The maximal evolution times in the indirectly detected dimensions were set to 50 ms for 15N, 10 ms for 13C and 20 ms for 1H.
The spectral widths were set to 12,500 (acq) × 2000 (15N) × 8000 (13Cali) × 6250 (1Hali) Hz and maximal acquisition times in the indirectly detected dimensions were set to 50 ms for 15N, 10 ms for 13Cali and 16 ms for 1Hali.
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Overall, 512 complex data points were collected in the indirectly detected dimension (13C) with 4 128 scans and 2048 points per increment.
Simulations with up to 2 million coupled volume elements (256 x 256 x 32) show that iZQCs can significantly narrow linewidths in the indirectly detected dimension of systems with inhomogeneous fields and explore the effects of shape and orientation of the inhomogeneities.
The phase sensitive adiabatic HSQC (Heteronuclear Single Quantum Correlation) [24 26] with multiplicity editing spectra were acquired with an acquisition time of 0.2 s, relaxation delay of 1.0 s and spectral windows of 4500 6000 Hz in F2 and 20,100 21,100 Hz in F1. 512 complex data points were collected in the indirectly detected dimension (13C) with 2 8 scans and 2048 points per increment.
The temperature was set to 300 K and the spectra were recorded with 2048 complex points in the directly acquired dimension and 512 points in the indirectly detected dimension.
Nonrefocused 2D H-C HSQC spectra were acquired without decoupling and refocusing in the directly detected dimension.
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