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Moreover, in artificially infected fish, the detected copy numbers of the N gene were much higher than those of the G gene in all tissues, and both the N and G gene copy numbers were highest in the kidney and spleen.
This implies that the detected copy number is, in fact, dependent on the real-time PCR assay performed.
This can be observed clearly in fig. 1A, Assay 3: In DNA of less than 300 bp, the detected copy numbers for 18S were strongly reduced, leading to a considerable deviation from the correct SRY-to-18S ratio of 1∶2 in fractions of DNA <500 bp.
HGMEC: Detected copy number in HGMEC.
A particularly important feature of our method is to estimate the clinical significance of detected copy number changes.
This may be due to the fact that total 84 olfactory receptor genes were included in the detected copy number variable genes.
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The detected copy-number abnormalities are detailed for each cell line in Tables S2 and S3.
The box-plot graph used to compare the number of detected copies of the genes was done using EXCEL software (Microsoft® EXCEL 2000).
Therefore, we did not know if a detected copy-number increase or decrease represents a derived or ancestral allele.
As our sample from which we detected copy-number variants consists of three African lines and the non-African reference strain, we use the demographic model of Li and Stephan (2006) as one of our null models.
The number of detected copies of each BoERV family was variable.
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