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The box-plot graph used to compare the number of detected copies of the genes was done using EXCEL software (Microsoft® EXCEL 2000).
However, the limit of low-copy number detection decreased to somewhere between 800 (which could be detected) and 80 (which could not be detected) copies per reaction when a TaqMan® DNA hydrolysis probe was used to monitor amplification.
The number of detected copies of each BoERV family was variable.
The total number of the strong hits was only 6% of all detected copies, but their size reached 42%.
Interestingly, the opening ATG codon was conserved in all detected copies of both families, including conservation of this codon at both ends of all copies of Orly.
The presence of unidentified nucleotides in some of the detected copies in D. mojavensis suggests the possibility that other sequences are potentially full-length copies.
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The detected copy-number abnormalities are detailed for each cell line in Tables S2 and S3.
This implies that the detected copy number is, in fact, dependent on the real-time PCR assay performed.
HGMEC: Detected copy number in HGMEC.
Therefore, we did not know if a detected copy-number increase or decrease represents a derived or ancestral allele.
A particularly important feature of our method is to estimate the clinical significance of detected copy number changes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com