We also examined lysates with non-denaturing PAGE (left panel in Fig. 2C) and size exclusion chromatography (SEC; supplementary material Fig. S1), in which the Aβ-MRF was detected as a complex with 10 15 monomers and monomers and low-n oligomers were not detected.
Candidate subgraphs with highest scores are detected as conserved complexes, using a similar heuristic as in [ 78].
All lineages suspected from dendrogram-based analysis were also detected as clonal complexes in the MST, including the newly described lineages.
In addition, given that proteins within a protein complex interact with each other to perform a common biological function, the algorithms should also focus on ensuring that the protein members detected as protein complexes have high functional homogeneity.
Pathological foci of mastitis were detected as well in anterior complexes as in posterior complexes, but severe changes were more present in the latter [ 20].
Subsequently, {4, 5, 6, 7} is detected as another putative protein complex from this sample PPI graph.
First, analysis of cleavable complexes (detected as DNA double-strand breaks) by pulsed-field gel electrophoresis confirmed the correlation between cleavable complex formation and cytotoxicity of three topoisomerase-targeting drugs in HeLa S3 cells (the order of effects being VM26 > m-AMSA > VP16).
All sera were diluted 1 200 and separately tested for reactivity against each peptide and immune complexes detected as described above.
Mutation of C271 (C271A) stabilizes the covalently linked protein-RNA catalytic intermediate, which can be detected as higher-molecular-weight complexes by western blot (Hussain et al., 2009).
When we carried out GO clustering of the single human network, the DAB complex was detected as part of a much larger cluster consisting of 23 proteins, of which only nine are present in the real complex (results not shown in Fig. 4).
