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The riboprobe sf6 yielded 163 bp of sf6 and 46 bp of exon5, present in most BORIS isoforms (Fig. 5H) The C6 riboprobe detected alternative (185 bp) and originally-described (85 bp and 75 bp) splicing in ZF region of BORIS (Fig. 5I).
We also detected alternative splicing events in our data.
Most of the detected alternative transcripts (22/27) harbored premature termination codons (PTCs).
Are proteins produced from all of the detected alternative transcripts, in particular transcripts encoding rare splice insertions?
Functional enrichment analysis once again detected alternative splicing as the top enriched term (Table 1, red color and Additional file 10: Table S10).
Northern blots and sequencing revealed that the microarray probe targeting the 3' end of the mRNA molecule detected alternative splicing of the last exon and not differential expression.
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This optical architecture could be optimized for detecting alternative important wavelength signal, such as 1550 nm.
A modified approach was designed to cluster and assemble large EST datasets, aiming to detect alternative splicing together with EST abundance of each splicing variant.
Appropriate experimental designs for detecting alternative states are discussed, and recent experimental studies in coral reefs, rocky intertidal systems, soft-sediment beds, and subtidal systems are briefly reviewed.
CT can identify the presence and size of stones with a very high accuracy of >95%% and is able to detect alternative diagnoses that simulate renal colic in 5 10 % of patients [16, 17].
Next-generation sequencing has also been employed to identify recombination events occurring within the genomes of higher organisms, for example, detecting alternative RNA splicing events and oncogenic chromosomal rearrangements.
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