Exact(2)
In the three specimens with detectable sequences, we noted that the copy number was very low as nested PCR and replicate testing was often needed for detection.
A typical Pfam-A entry contains a seed alignment, an alignment of a representative set of sequences, an HMM built using the seed alignment, a full alignment of all (detectable) sequences in the family and a description of the family with additional details such as the threshold parameters used to create the full alignment.
Similar(58)
While their method was powerful, it was limited to the detection of recent HGT events since statistically detectable sequence similarity fades over evolutionary time15.
Their work should be very sensitive at detecting homologs, while our novel MBOMP predictor has the potential to detect analogs or distant homologs with no detectable sequence signal remaining.
Therefore, these analogous enzymes cannot be detected by classical comparative genomics approaches, as they do not share any detectable sequence similarity.
Thus, two proteins that share no detectable sequence similarity can be discovered by structural techniques to have the same fold, and are most likely homologous.
Furthermore, despite a lack of detectable sequence duplication, the structure incorporates two topologically analogous domains related by pseudo two-fold symmetry.
The extent of sequence diversity within each infection sample compared to the local population diversity was assessed using the FWS fixation index, which has a potential range from zero (where an infection contains as much diversity as the total seen across the local population) to 1.0 (an infection containing no detectable sequence heterogeneity).
Since all five of these proteins (two in SFV1, two in AFV1, and one in SIRV2) appear to be homologs, the lack of any detectable sequence similarity between, for example, SFV1 VP5 and the other four, suggests that there must be a large number of other sequences sharing the same fold that exist in archaeal viruses that have yet to be discovered or characterized.
Latexin, the only known mammalian carboxypeptidase inhibitor, has no detectable sequence similarity with plant and parasite inhibitors, but it is related to a human putative tumor suppressor protein, TIG1.
We then describe how we have used structure-based modeling to show that several other viruses with no detectable sequence similarity are likely to have coats constructed from similar proteins the "double-barrel trimer".
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