We analyzed at least 50 independent primary transformants for each construct in wild-type and sub-1 backgrounds and continued with lines that showed detectable root signal for further analysis.
The absence of detectable root-colonization by Acaulospora could be due to incompatibility with common beans, or difficult staining of the root-colonization structures [ 34], or that staining of Acaulospora root colonization is tricky, it may be a false negative, since some Acaulosporas are difficult to stain with the blue dye [ 35].
EsDREB2B transcript was detectable in roots, stem and leaves of unstressed (i.e. control) seedlings, and was most abundant in leaves (Additional file 3: Figure S3).
Both proteins were predominantly expressed in the vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems.
The transcript of OsHsfC1b was not detectable in roots of the ami-7.1 line.
Arabidopsis ARAF1 (At3g10740 or ASD1) is similarly broadly expressed, with ARAF1 transcripts detectable in roots, rosettes, stems, flowers, and siliques [ 97].
Under control conditions, GFP expression was readily detectable in roots of GFP-mir168* plants whereas only some fluorescent spots were visualized in their leaves, mainly at the distal areas.
Although transcript of TCL2 is detectable in roots, all the mutants tested including TCL2 overexpressor, TCL2RNAi and P TCL1 :TCL2/tcl1 are largely indistinguishable from wild type in term of root hair formation (data not shown).
Of interest, GFP was readily detectable in roots of pMIR168a::GFP plants that have been treated with fungal elicitors indicating miR168 promoter responsiveness to fungal elicitors, at least in this organ.
The HvFIE mRNA is abundant in shoot meristems, young shoots, pistils before fertilization and seeds of 1-2 Dandand 3-5 DAF, whereas it is hardly detectable in roots, leaves and stamens.
