For the analysis of Ki67, the detectable ratio was 0.644 and so the study was powered to detect a difference between changes of, for example, -75% and -91%.
Table 1 shows the detectable ratio of ssDNA_ODN from positive control.
If an abundant gene has an intensity of 1000 in another scanning channel, the largest detectable ratio will be 65.
On the other hand, the phylogenetically distant species Arabidopsis shows a barely detectable ratio of percent hits to database extent (data not shown).
After adjusting for age, sex, total cholesterol, current smoking and race, MetS was not associated with prevalence of CAC, but it was associated with a higher level of CAC if detectable (ratio of geometric means of CAC levels 1.55) (Table 5).
In many cases, particularly for phenotypes suggestive of moderate to large odds ratio, this difference in detectable odds ratios will not change of the overall outcome of the association study.
Regarding the results shown in Table 3, we can emphasize that the replay attack is the most detectable scenario, with a detection ratio of about 40%.
Using a ratio of each gene signal to that of the trfA signal, the following groups could be assigned: (1) Genes that were readily detectable had ratios from 1.0 – 0.2; (2) genes that were barely detectable had ratios of 0.2 – 0.02; and (3) genes that were not detectable had ratios of less than 0.02.
The effect of the CV on the minimally detectable expression ratio (threshold expression ratio) was iteratively estimated for a power of 0.9 and α = 0.01 using the sample size estimate procedure for a two-sample t-test.
For our response phenotype in the discovery set, we had 80% power to detect a minimum detectable odds ratio of 1.9 assuming an allele frequency of 0.05 and 5% significance level using our Caucasian sample.
