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Four purified FP6s were obtained, which had the same molecular mass and different isoelectric points without any detectable modification in the course of purification.
Retarded protein DNA complexes were observed using the three distinct GC-box oligonucleotides and TSA treatment did not induce detectable modification of the observed pattern of retarded protein-DNA complexes.
Taken together, our results indicate that the FACS sorting procedure does not introduce any detectable modification of NB properties.
Despite profound effects on flies exposed to toxins or mutations directly or indirectly affecting cytochrome oxidase (Fernandez-Ayala et al. 2009; Kemppainen et al. 2014), or even the pleiotropic phenotypes caused by partial knockdown of DNA polymerase γ (Humphrey et al. 2012), AOX expression produced no detectable modification to the tko 25t phenotype.
In healthy volunteers, injection of contrast material did not produce any detectable modification in the lung tissue volume calculated using Lungview®, probably because the contrast material remained strictly intravascular in the presence of an intact alveolar capillary barrier and was rapidly eliminated in the urine [ 2].
Data obtained with the activation of the DNA damage checkpoint by etoposide provide an example of detectable modification of cell cycle suggesting that Capan-2 Fucci expressing spheroid models could be used to detect or to investigate the effect of a novel DNA damaging compound.
However, it is in contradiction with the lack of detectable modification of the brain proteome of Prnp-/ mice [ 29], which could rather suggest that the variations observed in the miRNA profile is a consequence of the scrapie infection rather than of a PrP loss of function.
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No detectable modifications of pulmonary parenchyma were present in the histology of wild-type, vehicle-, or control siRNA-treated mice (Fig. 3A, a1, a2, a3).
The detectable effective modification depth estimated from the depth profiles is 250 300 nm at an ion energy of 50 keV and 400 450 nm at an ion energy of 180 keV.
Two TEM samples that were prepared from the opposite sides of RB-QD04-0008 do not show any detectable surface modification.
Thus, treatment of cells with serine proteinases can affect integrin-mediated attachment to matrix proteins in a manner moderated by Ca2+, but the alterations in attachment do not appear to be accompanied by detectable proteolytic modification of the integrin.
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