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The paternal genome of the apparently haploid progeny of crosses to ms(3 K81 /ms(3 K81 males was nearly universally excluded: we observed a single interesting and readily detectable exception among >150 sequenced haploids.
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After hospitalization ARE are no longer detectable with the exception of MT159 which was able to persist.
In 22-month-old mice that did not have structural cartilage defects, HO-1 staining in articular cartilage was no longer detectable, with the exception of a few cells at the joint margin (Fig. 2a).
All 160 miRNAs were detectable with the exception of c-lin-4 in FFPE and c-lin-4, mir-104, mir-122a, mir-144, mir-302b and mir-325 in snap frozen sample.
Specifically, their expression in the carcinoma cell lines (1A9 and SKOV3 ovarian, MDA-MB-431 breast and HT29 colon) was not or was barely detectable (with the exception of PRSS3 by the HT29 colon carcinoma).
All epitopes fused were detectable with the exception of those from CP-His and 2C-NΔ24-CP, which may have been caused by protein misfolding or the epitope sequence, e.g. in case of 2C, being trimmed in size too much and thereby not being recognized anymore by the antisera.
Indeed, organogenesis generally proceeded without detectable abnormality, with few exceptions.
Autoantibodies to oesophageal cytokeratins were, with few exceptions, barely detectable.
In the few exceptions without detectable neurological symptoms MS-like neuropathology was nevertheless found.
These analyses revealed that the detectable differences were (with one exception) lower than the 20% effect size that is usually set for regulatory risk assessment studies [37].
All but one of the mutated plasmids expressed no detectable β-galactosidase activity; the exception was where the ATG was converted to the usually less efficient start codon GTG (pBIO1928), in which the expression of the translational fusion was much-reduced, but not abolished (Figure 3C).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com