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These two patients had no detectable compound muscle action potential on electrophysiological examination.
The enzymes used as bioreceptor components in biosensors are usually proteins of oxidase type that can selectively react with specific analytes, consume dissolved O2, and produce H2O2 that is an easily detectable compound.
In a phase I trial of I3C, DIM was reported to be the only detectable compound and reached a level of 607 ng/mL (2.46 μmol/L) after the administration of a single dose of 1000 mg [ 41].
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Here, we present NTFD (non-targeted tracer fate detection), a software for the non-targeted analysis of all detectable compounds derived from a stable isotope-labeled tracer present in a GC/MS dataset.
Even though castrated male urine contained 10 detectable compounds, the five male specific compounds present in intact males were absent in castrated male mice urine.
Given that there are slightly over 260 compounds in the serum-metabolome database (SMDB) with normal concentrations >1 µM, one might have expected that the GC-MS detectable compounds would have been much higher than 90.
Of the detectable compounds, 9 fatty acids were identified by both targeted and global GC/MS analysis, while 2 were unique for the global analysis (capric acid and arachidic acid) and 16 were unique for the targeted approach.
However, dilution equal or beyond 3c brought the concentration of detectable compounds below the minimum sensitivity threshold of optical spectroscopy.
No other detectable compounds had retention times or signals that caused interference with the δ-ALA peak.
First, a manual or automated peak picking is performed, which provides a list of all cross-peaks of all detectable compounds in the sample.
Detectable compounds are limited to those that can be derivatized, which can be time consuming, costly, and carrying a risk of metabolite loss.
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