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Here, we demonstrate that ablation of c-RAF expression in advanced tumors driven by KrasG12V/Trp53 mutations leads to significant tumor regression with no detectable appearance of resistance mechanisms.
Furthermore, accumulation of ssDNA preceded the detectable appearance of DSBs, suggesting that ssDNA formation at replication forks is a precursor to chromosome breakage at those locations.
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Whereas Golgi and ER were easily detectable by appearance in control photoreceptors (Fig. 4E, inset for second example), neither compartment was morphologically identifiable in Sec13-suppressed photoreceptors (Fig. 4F, inset for second example).
The forward primer designed for amplification of the Actin2 was intron spanning; thus, genomic DNA contamination in the sample was detectable by the appearance of a DNA band at 291 bp.
Treatment was initiated with one spot for 60 seconds' duration at a power setting ranging between 360 and 880 mW such that no visible change or a barely detectable light-gray appearance to the lesion was present at the end of the treatment.
Interestingly, the time lapse between the detection of the PFSPs and the appearance of detectable pSer129-reactive inclusions suggests that some populations of PFSPs remain below detectable levels or that the PFSPs were able to initiate the seeding or slow formation of αS inclusions before being degraded.
Moreover, the same location could be imaged on separate occasions with no detectable change in the appearance of the fluorescence or reflectance images.
Model experiments suggest that the appearance of detectable concentrations of this radical result from a disturbance of the normal state of the ascorbic acid, dehydroascorbic acid redox system.
This differential adaptive capacity likely underlies the more rapid appearance of detectable skeletal changes in OA in comparison to the articular cartilage.
In three different experimental paradigms (orthotopic tumor cell line allografts, tumor transplantation, and de novo tumor development), the appearance of detectable MMTV-PyMT mammary tumors is significantly delayed in the NG2 null mouse.
A complete response (CR) required complete disappearance of all previously detectable disease with no appearance of new lesions, and partial response (PR) required at least a 50% decrease of the sum of the products of the two greatest perpendicular diameters of all measurable lesions, with no appearance of new lesions or progression of any lesion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com