For both analytes, the method enjoys wide dynamic range (1 50 μg ml− 1) with good detectability (limits of detection 0.11 μg ml− 1).
This primer extension assay was then validated in terms of linearity (linear range = 0.5 2.5 nM), detectability (limits of detection and quantification = 0.023 and 0.091 nM, respectively), and precision (total precisions = 8.1% and 3.7% for dATP and dCTP, respectively, n = 9).
However, the lack of AMP detectability could either indicate instability or amounts below the detection limit, both valuable reasons to exclude the plant lines from further studies.
By tracking this variability, we were able to identify and select bacterial peptides that were below the DDA detection limit through the dilution series and test the ability of SRM to expand the range of detectability in a mixture of proteomes.
It should be also noted that the not detectability of siccative oil in the other samples is due to the small sample size which gave signals at the detection limit.
Compared to these, the detection limits of VSNEA in this study show superior detectability.
This system was operated in the positive ion mode with a detection-limit of 10 nM for the MMP reaction product and provided 60 folds enhancement of sensitivity by using selected reaction monitoring detection compared with MS full scan mode, which significantly increased the detectability of the system and therefore reduced the enzyme reaction time in both off-line and in-line mode.
Generally, detectability was limited by detector noise performance.
The limit of detection (detectability), detection index, broadening index, modified contrast index, densitometric visualizing index, and linearity range were determined and tested for estradiol, tocopherol, tocopherol acetate, ibuprofen, stearic acid, stearyl alcohol, and salicylanilide detected by the use of visualizing reagents.
