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The pathways in ocsESTdb were classified into two types to detect the functions of lipid metabolic pathways for oil crop seed unigenes.
It is particularly useful for revealing whether the strongest co-expressed gene pairs constitute the most promising candidates for experimental assays to detect the functions of uncharacterized genes.
Finally, we used the tube formation assay to further detect the functions of As2O3 in the angiogenic ability of prostate carcinoma cells.
To detect the functions of csRNAs, we searched for the transcripts of the coding genes that are complemented by the csRNAs responsive to heat stress.
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Uptake of fluorescent DiI-Ac-LDL and microtubular formation in three-dimensional cultures were used to detect the function of LECs.
Other investigators prefer the somewhat simpler approach to detect the function of the MGMT gene by means of immunhistochemistry [14] [16].
The maximum lumbar flexion-extension radiographs in standing position are considered to be a reference standard to detect the function of the passive stabilization system [ 6, 7].
We selected stable cell clones constitutively silenced by sh-PAX8 to detect the function of this transcription factor in an epithelial ovarian cancer cell line.
In this study we investigated the specificity and sensitivity of the fluorescent probes rhodamine 123 (R123), daunorubicin (DNR) and calcein acetoxymethyl ester (calcein-AM) in order to detect the function of the drug transporters P-gp and MRP, using flow cytometry.
We further detected the function of PcNLPs protein in vitro based on the site directed mutagenesis of four amino acid residues in a conserved motif.
We also detected the function of ZD55-AChE in the pancreatic cancer cell line BxPc-3, liver cancer cell line Huh-7 and SMMC-7721, and colon cancer cell line SW480 and HCT116, and found that ZD55-AChE inhibited BxPc-3, Huh-7 and HCT116 cell growth at a MOI of 1.
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