Semi-quantitative PCR was used first to detect the expression of testis/ovary specifically expressed genes.
Northern blots were further performed to detect the expression of miRNAs.
However, we did not detect the expression of OsPIN1b at the very early stage of LRP development (Figure 3D1).
Next, immunohistochemistry was performed to detect the expression of COX-2 protein in 88 prostate cancer tissue samples.
To detect the expression of St14 in both pluripotent stem cells (PSCs) and MEFs, semi-quantitative PCR and immunoblotting were performed (Fig. 1A and 1B).
To our knowledge, there have been no previous reports of using GNR-based SERS as a tool to directly detect the expression of inflammatory molecules on inflamed macrophages.
To detect the expression of inflammatory genes of monocytes, similar methods were used as described in the original study by Padmos et al. (2008).
In this case, a FITC-ICAM-1 antibody was used as an indicator to detect the expression of ICAM-1 on the surface of Raw264.7 cells.
Transcription analysis was performed to detect the expression of the ncRNA31 gene in the deletion mutant and the wild-type strain A1501.
We also used this monoclonal antibody to detect the expression of LRRC25 in peripheral leukocyte subsets including T cells, B cells, NK cells, monocytes, and neutrophils without permeabilization.
Eventually, supernatants were collected to detect the expression of IFN-γ and IL-17.
