Sentence examples for detect the efficiency of from inspiring English sources
The red star indicated the conversion of C to T. To detect the efficiency of base editing, the region around the target sites was amplified and analyzed initially by the T7EN1 cleavage assay.
To detect the efficiency of bacterial escape from the phagosome, cells infected with the L. monocytogenes strain NF-L327 were spun and fixed as previously described.
We used high-resolution electrospray ionization Fourier transform ion cyclotron resonance (ESI FT-ICR) mass spectrometry to confirm protein primary structures and to detect the efficiency of coupling maleimide into Avd(S16C).
Moreover, pEGFP plasmid was transfected in equal amounts in both of the previous transfections in order to detect the efficiency of DNA intake (Additional file 1: Figure S1).
PCR primers used to detect the efficiency of recombination of Fbw7 and R26 alleles are given in the Supplemental Experimental Procedures.
Afterwards, a qRT-PCR with a dilution series was performed to detect the efficiency of the qRT-PCR reaction and to prove that only one product can be detected within the melting curve.
In our initial study, we tried to optimize the model procedure mentioned above by detecting the efficiency of different reaction conditions, such as piperidine/EtOH, p-TSA/EtOH, AcOH/EtOH, AcONa/AcOH, H2SO4/AcOH, DBSNa/MeOH, DBSNa/EtOH, DBSNa/H2O, DBSNa/AcOH (Table 2).
In this paper, we use HAM to detect the fin efficiency of convective straight fins with temperature-dependent thermal conductivity.
These clones were used for the subsequent experiments In order to detect the silence efficiency of shRNA expression vector targeting XTLY-I gene, real-time PCR was performed to detect the mRNA expression.
The lentiviral vector pGCSIL-GFP contained a U6 promoter to express continuously the shRNA and CMV promoter to express the green fluorescent protein (GFP), which could be used to detect the transfection efficiency of viral packaging and infection.
To detect the silencing efficiency of specific genes, the isolated total RNA was reverse-transcribed into cDNA using the M-MLV Reverse Transcriptase (Promega, USA) with an oligo (dT 18 primer.
