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There was full agreement that the antibodies used for detection of EBV antigens in multiple sclerosis lesions are suitable to detect staining of EBV-infected cells in infected control tissue.
We did not detect staining under these conditions.
This makes it difficult to detect staining especially in this area.
The P33/37/41-phospho-β-catenin antibodies also detect staining at centrosomes and midbody bridges in mitotic cells and in the nucleus (Fig. 1B and C).
We were not able to detect staining in layers IV and V. High magnification photographs revealed that P-ERK1/2 was detected in both dendrites and cell body (figure 4C).
We were unable to detect staining for IL6 or SPRY1.
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Immunostaining is much more sensitive than heme staining, and very short exposure times were sufficient to detect stained bands on the nitrocellulose.
This method detects staining of cell bodies, fibers and punctate structures.
Other authors detected stained sentinel nodes only in a minority of their patients (Stoeckli et al, 2001).
One-step silver staining was used to detect silver-staining nucleolar organizer regions.
Sense probes did not detect reproducible staining (data not shown).
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