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To better detect NEXT accumulation, we treated ANGPTL2-stimulated human endothelial cells with the γ-secretase inhibitor, DAPT to block S3 cleavage to allow NEXT buildup.
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Because osteoclasts were detected next to the characteristic outlines of the substitute particles, it is suggested that BCP is resorbed by osteoclasts.
Furthermore, COMP staining was also detected next to the cells in the pericellular space associated with its fine fibrillar material.
In addition, remaining vessels with a relevant perfusion rate could be detected next to the treated region in the arterial phase of the CT scan.
Degradation products with molecular masses around 50 kDa could be detected next to the original compounds with molecular masses up to 100 MDa.
The latter family together with its YgiU-like counterpart is present mostly in phages and prophages of Firmicutes, whereas the gepA gene was detected next to a genomic region enriched in TA genes in Dichelobacter nodosus [ 74].
Increased BrdU incorporation in DNA is detected first, at 24 h, as cells progress through the S phase of the cell cycle; 30 and MTT conversion is detected next at 48 h as a measure of metabolic activity related to the G2 and mitosis phases of the cell cycle.
Prospective methods are real time methods, where analyses are repeated at regular time intervals using early detection systems to detect the next outbreak [ 3].
Dr. Hughes said his agency suspected that an alert doctor would be the first to detect the next outbreak, if one occurs.
"We want to keep focus on the questions we've been asking, but [also] how we can be in position to detect the next thing," said Stephanie Pfirman, an Arctic scientist at Barnard College in New York City, who co-chaired the report.
Therefore sequencing errors are easier to detect in next-generation sequencing read alignments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com