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They demonstrated that this method can increase detection accuracy at a reduced sequencing cost by applying their model to detect modifications in plasmids with known modified sites and an E. coli K-12 strain.
To achieve this goal, we used quantitative real-time PCR (qRT-PCR), which is the technique of choice to detect modifications in the level of transcription of specific genes in a reliable and reproducible manner.
By contrast, in patients with right hemisphere impairment we did not detect modifications in corticospinal excitability during any of the grasp type observed, plausibly because the dominant hand (i.e., left) is unaffected and already involved in daily activities.
Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis.
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In summary, voxel-based morphometry can detect modifications of visual pathways structures mainly in advanced stages of glaucoma.
If measured CrCl must be ideally used to detect modifications of the renal function, in clinical practice, age, reason for admission, and CKD-EPI could be used as screening tool to identify ARC.
However, the ability of the DI in detecting modifications of TMS-evoked potentials due to physiological and/or pathological alterations in cortical circuits should be carefully evaluated.
Using an anti-ser 3 p-cofilin antibody, previously reported to allow for detection of subtle changes in cofilin phosphorylation [34], we could not detect significant modifications in the levels of phosphocofilin after mrTIMP-1 treatment either by western blot (Fig. 3E) or by immunofluorescence (results not shown).
According to the EMI technique, diagnosis and prognosis can be carried out to detect structural modifications in an operative state.
A coimmunoprecipitation experiment was performed to detect potential modifications in actin binding to villin during the course of infection.
Considering that we cannot reliably detect m5C modifications in our setup, it is possible that M. Cdi633is an m5C specific methylase.
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