For worm images where only the nuclei of cells are lit up using fluorescent labels, it is often hard to detect a clear worm boundary, except for the cases to be discussed in Section 3.2.
15, 16 The small number of Mf carriers in the field study does not detract from the results; the card test and test strip detect an adult filarial worm antigen in human blood, and they are not designed to detect Mf.
At the genomic distance of 1.2 MB we did not detect a significant change in distances in smc-4 RNAi) smc-4 RNAiNAi) worms compared to vector control worms.
Once the worm was detected, a tweak to the numeric online address for the White House Web site prevented disruption.
Western blotting from L4 worms also detects a drop in APL-1 protein expression on the unc-104 e1265) unc-104 e1265igure 4D, E). qRT-PCR comparing apl-1 expression background and unc-104(e1265) backgrounds showed no diFigureces, suggesting that the loss of APL-1::GFP is occurring at the protein level (Figure 4D).
Consistent with the DAPI studies, in N2 worms we detected a dramatic increase in mean nuclear genome copy number at 10 12 days of age (t-test 4 days vs. 14 days, P < 0.0001) (Fig. 5a), the same point in middle age where the DNA masses were visualized.
Microsoft has had a software patch available for weeks that allows system administrators to correct the problem, and major computer security companies have offered their own versions of programs to detect the worm, eradicate it, and install the patch.
The vendors of antivirus programs, like Symantec and McAfee Security, have posted on their Web sites updated antivirus definitions and removal tools that can detect the worm and clean infected computers.
To detect endogenous worm agrin by western blotting young larval stages were homogenized in ice-cold RIPA buffer (NaCl 150 mM, TrisHCl pH8 50 mM, NP-40 1%, deoxycholic acid 0.5%, SDS 0.1%, NaF 50mM) containing a cocktail of protease inhibitiors (Complete Mini EDTA-free, Roche).
We present two algorithms, BDB− and BDB+, to detect the worm backbone under different scenarios.
