Yet we did not detect a requirement for any of these MBONs in short term aversive memory when tested individually.
In principle, by comparing identically treated control and ABKO mice, we should detect a requirement for β-catenin in proliferation or survival as a relative decrease in the EYFP labeling indices of ABKO pancreata compared with those of controls.
We were unable to detect a requirement for TULP3 or BBSome components, although these are essential for ciliary localization of SSTR3 and MCHR1 (Berbari et al., 2008b; Jin et al., 2010; Mukhopadhyay et al., 2010).
We also did not detect a requirement for Arf4, ASAP1, or Rab11, which are essential for rhodopsin delivery to the rod outer segment (Deretic et al., 2005; Wang et al., 2012).
Whether or not we detect a requirement for a particular MBON in a particular learning paradigm is likely to depend on which DANs are recruited by the US used in that paradigm as well as the degree of redundancy in the MBON representation of valence.
While we did not detect a requirement for the GABAergic MBON-γ1ped>α/β in appetitive memory, previous study have shown that dopamine input to the γ1 and pedc suppresses expression of appetitive memory in fed flies (Krashes et al., 2009 ), indicating some role of MBON-γ1ped>α/β in appetitive memory.
The protocol was designed specifically to minimize confounding factors that could diminish the power to detect a difference in insulin requirements during the two 18-h periods of closed-loop glucose control after the HF and LF dinner meals.
To detect a reduction of transfusion requirements of at least 2 ± 2 units after FBNc administration, with an 80% power (β-error) and a 95% confidence interval (α-error) for the nonparametric Wilcoxon rank sum test, data from at least 60 patients per arm (comparator and FBNc) would be needed.
Important issues for these experiments were the high accuracy and position requirements necessary to detect a possible failure wave or failure kinetics in SiC-ceramics as might be evidenced by a change in the slope of the penetration velocity impact velocity curve.
In this paper, we provide a novel engineering framework, consisting of a modelling language and nine analytical reasonings, which is meant to represent transparency requirements and detect a set of possible side-effects.
