There are no records of the initial causes of or responses to such altered genetic codes; further adaptations to and details of eventual fixation are completely unknown.
Details of embryo fixation and immunostaining, live imaging and superresolution imaging, and MitoTracker staining of embryonic mitochondria are provided in the methods in the supplementary material.
The variation in the proportion of each grade reported in the different studies can be explained by the variation in the grading system used and the difference in the patient cohorts, including age distribution, symptomatic versus screening population, early versus advanced breast cancer groups, and details of tissue fixation.
In separate work, we have also implemented a stochastic rather than a deterministic analysis of this model, and find that although some details concerning the timing of fixation change, the general result is not altered.
b Details of the final suture fixation.
Details of tissue collection and fixation have been previously described [ 24].
All other potential "true risk factors" that are patient related factors (e.g. gender, age, pre-existing diseases), puncture site and technical details of catheter placement and fixation (e.g. number of attempts until successful placement, catheter tunnelling) were removed as insignificant.
The Rottometer and details of the examination procedure and the fixation of the test persons to the device have been presented previously [ 13].
We investigated the case with the highest probability of fixation (one duplicate gene with two copies) in more detail in fig. 3 B. We got very similar results for a wide range of population sizes and deleterious mutation rates.
The initial osteotomy type, method of fixation, subsequent surgical attempts at achieving union prior to use of the IEF and details of adverse clinical incidents are recorded in Table 2.
