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The detailed protocols are available from the supplement (see additional file 1: detailed information on methodology).
More details on this experimental procedure can be found in the Methods sections and detailed protocols are given in Supplemental File S1.
All detailed protocols are listed by name and are documented and available at https://www.protocols.io/groups/sullivan-lab.
A list of instruments and detailed protocols are provided together with EM-specific reagents required for sample preparation.
The time elapsed between measurements in vivo and fibroblast donation, as well as detailed protocols, are indicated in Methods S1.
Similar molecular methods were followed for each of the three nuclear gene fragments (detailed protocols are found in the works cited above for each marker).
Similar(15)
The resultant sample is not nationally representative but detailed protocols were designed to allow future replication.
An outline of this approach and a detailed protocol are provided in Supplemental Experimental Procedures.
A full list of the in situ hybridization probes and a detailed protocol are available upon request.
The detailed protocol is outlined in Supplementary Method 9.
The detailed protocol is provided in Supplementary Information.
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