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Total RNA was extracted according to the detailed protocol provided by TRIzol reagent manufacturer (Life Technologies).
Use detailed protocol provided in Protocol 1, as well as in the reference (Bell et al., 2007 ).
Protoplast isolation and transformation 1. Prepare protoplasts from two plates according to the detailed protocol provided in Roberts et al. (2011) through step 3.3.11 using a P. patens line in which your gene of interest is knocked out.
Campbell-Switzer Silver Stain was done according to a detailed protocol provided by Dr. Switzer (NeuroScience Associates, Knoxville, TN; Campbell S, Switzer R, Martin T (1987) Alzheimer's plaques and tangles: a controlled and enhanced silver staining method.
Embryonic pancreata were dissected and immediately placed into extraction buffer containing a protease inhibitor cocktail (0.5 mg/L TPCK, 0.5 mg/L TLCK, 0.6 µM leupeptin, and 2 µM pepstatin), DTT, and PMSF (detailed protocol provided upon request).
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Each guideline consists of (1) a checklist specifying the data elements of the specimen and tumor that should be included in the diagnostic pathology report, as well as the valid values that these data elements may take and (2) a detailed protocol providing definitions and further information about the scientific basis for assessing these variables.
The detailed intervention protocol provides a standardised approach to intervention design, delivery and content for use in diet, physical activity and weight loss trials.
A detailed protocol is provided in the Methods S1.
A detailed protocol is provided in Methods.
The detailed protocol is provided in Additional file 1 : Methods.
A more detailed protocol is provided in Additional file 1.
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