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The ER Calux assay with stably transfected T47Dluc human breast cancer cells was developed by Legler et al.[72] and was conducted in this study according to the detailed protocol given in Maletz et al.[84].[84]
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Assay design and detailed protocol have been previously described [17].
A detailed protocol is given in Supporting Methods S1.
More details on this experimental procedure can be found in the Methods sections and detailed protocols are given in Supplemental File S1.
The reconstruction process has been reviewed conceptually in literature [ 17- 22] and, recently, a standard operating protocol giving a detailed overview of the necessary data and steps has been published [ 23].
The published protocol gives a more detailed account of the methods.
A list of the sources of reagents and equipment along with a detailed protocol for EADSA is given in Ref. [12].
Secondly, we will focus on the use of this approach to monitor translational activation and repression in Xenopus oocytes, giving a detailed protocol, and discussing possible optimizations we have explored.
Trained interviewers, mainly local CDC staff members, were given a detailed protocol for the survey, conducted face-to-face interviews in private rooms at the CDC or the local MSM community based organizations (CBOs).
A detailed protocol is described on the website given in Ref. [ 44].
A detailed protocol to determine the incoupling time has been given by Vogels et al. [24].
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