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The detailed procedures described herein promise to accelerate the usage of CRISPR/Cas9 system for genome editing in sea urchin embryos.
We measured Se using HG-AFS according to detailed procedures described elsewhere (Chen et al. 1999).
The detailed procedures described above are easily reproducible on the bench on condition that some special precautions are taken.
From ten patients, cells from bulk tumor were enriched for mammospheres according to detailed procedures described in Additional file 1.
Use the detailed procedures described in our user guide to reproduce the results described in the example: http://wiki.reactome.org/index.php/Reactome_FI_Cytoscape_Plugin.org/index.php/Reactome_FI_Cytoscape_Plugin
However, because these components refer specifically to the detailed procedures described in collaborative care/general practice models, this checklist could only be applied to trials in general practice settings [see Additional file 1].
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Real-time RT-PCR assay was performed according to the detailed procedure described by Ratchford et al. [16].
The data from the Q-PCR was analyzed by the 2– ΔΔ CT method [ 79], using the detailed procedure described previously [ 80].
The resultant alterochromide specific capture vector (pACR01) was linearized with BamHI and then used in TAR transformation with enzymatically (PmeI/ NaeI) digested P. piscicida JCM 20779 gDNA, following a detailed procedure described previously, to generate pACR02 containing the full cluster.
The pathway generation, pruning, and detailed analysis procedures described in this study can also be used to develop biochemical pathways for other commodity chemicals from gaseous substrates.
Incident squamous cell carcinoma (SCC) was identified through detailed assessment procedures described previously [ 21, 22, 27].
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