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Here we provide a detailed method for the design and preparation of geOMV displaying the O-antigen from a prominent uropathogenic Escherichia coli (UPEC) serotype, O25b, as a proof of concept for the use of geOMVs as vaccine candidates.
A detailed method for the radiosynthesis of N-succinimidyl-4-guanidinomethyl-3-125I]iodobenzoate (SGMIB) and its use for labeling internalizing molecules have been described in a previous publication [12].
Zhang et al. (2013) presented a detailed method for the estimation of straw-based power generation and concluded that the cost of straw-based power generation is indeed higher than the coal-fired power generation with life cycle analysis.
The detailed method for the pole test has been described previously [31].
The detailed method for the Morris water maze has been described previously [31].
The detailed method for the calculation of average daily alcohol intake has been described previously (Kropp et al, 2001).
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The detailed methods for the set of additional experiments are reported in the Additional file 1.
The detailed methods for the identification and chemical analysis of transformation products were described previously.
The detailed methods for the construction of the γ2-expression vectors and the protein purification are described in Document S1.
Please see the detailed methods for the in vitro cytotoxicity assay, including the dosimetry calculation for free drug mixtures and dual delivery LB-MSNPs, that are provided in the Supporting Information S2.
The detailed methods for the chemical analysis have been fully described by the Environment Agency (2007) and in other articles in which these data have also been examined (Johnson et al. 2007; Thorpe et al. 2006).
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