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In detail, tissue culture plates with the attached fibroblasts were washed with 1 ml of Dulbecco's phosphate buffered saline by careful rinsing to remove non-adherent cells followed by incubation for 75 min in a mixture of 750 µl RPMI 1640 with 10% FCS and 10 µl WST-1 '2- 4-iodophenyl -3- 4-nitro-phenyl -5- 2,4-di-sulfo-phenyl -2H-tetrazolium, monosodium salt'2- 4-iodophenyl -3- 4-nitro-phenyl -5- 2,4-di-sulfo-phenyl -2H-tetrazolium
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Detailed tissue architecture and cell morphology were visualised and identified in situ by FCFM.
We have constructed computational models of canine ventricular cells and tissues, ultimately combining detailed tissue architecture and heterogeneous transmural electrophysiology.
The advantage of these hybrid PET/MR systems is that they combine the strengths of MRI, i.e. morphological details, tissue characterization and spatial resolution, with that of FDG-PET, i.e., metabolic information.
Among the critical knowledge gaps needing to be bridged to aid in this effort include detailed tissue and cellular ontogeny of carcinogen metabolism and DNA repair enzymes, and associations of polymorphisms in DNA repair enzymes with childhood cancers.
Although histological staining could provide details tissue information, it still relies on ex vivo analysis on collected tissues.
In contrast, larger lepidopteran insects such as Manduca sexta and Bombyx mori allowed detailed tissue expression analyses of several neuropeptide GPCRs, which have helped elucidate novel functions of their ligands [10] [12].
However, more detailed tissue distribution analyses linked with the critical sites of prion action are warranted.
All participants signed written informed consent forms detailing tissue use for comprehensive experiments on breast cancer.
This dataset was derived based on GNF Atlas expression data [ 16] which details tissue expression patterns of genes across 79 different non-disease human tissues.
It will be interesting to extend detailed tissue specific comparisons to mouse/human embryonic stages to find out if distinctive tissue specific patterning is observed during prenatal development.
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