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We detail assay execution, including preparation of the experimental chamber and orientation of the MT against the barrier.
The PN assay is not only more sensitive than conventional MN and HI assays, but also allows us to dissect whether the neutralizing antibody responses act on virus release or virus entry or both (see Materials and Methods for detail assay description).
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Detailed assay procedure has been described elsewhere [46].
Additional file 1: Detailed assay protocol.
For each compound, compound details, assay details, target details and literature references are provided (Gaulton et al. 2012).
Detailed assay procedures are available at https://www.eurofinspanlabs.com/Catalog/Products/ProductDetails.aspx?prodId=0aCrd3Mu4RA%3d&prodId=0aCrd3Mu4RAclear=1.
Preanalytic performance, detailed assay characteristics, and clinical applicability of Ang-1 and Ang-2 immunoassays have not been reported.
A second focus should be to provide detailed, assay-specific direction on when results should be discarded; no clear guidance exists on the degree to which assays need to be re-run for failing to meet performance criteria.
Details of assays and inter-assay coefficients of variation are provided in Supplementary Table 1.
The details of assay rationale and design, instrumentation, pitfalls, data analysis, and further validation steps are described.
The details of assay characterization are provided in Text S1.
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