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The PSi layers were then detached from each substrate, in situ, by application of a high-current density step; complete detachment was achieved by subsequent immersion/rinsing in methanol.
By contrast, relatively high shear forces were required to detach P. agglomerans particularly from potato; under an applied shear force of 2.8 Pa only 9.5% detachment was achieved after 30 s.
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Under these conditions detachment phenomena were quite rapid with in most cases complete detachment being achieved over timescales of the order of 30 s.
Further refinement of our understanding of how intimate detachment is achieved may also aid the application of this unique aspect of mindfulness methods within various psychological therapies.
Detachment of cells was achieved with 10 mM EDTA treatment, followed by centrifugation and resuspension of the pellet in mitochondrial isolation medium (1 mM EDTA, 10 mM HEPES, 250 mM sucrose, pH 7.4).
Detachment of single cells was achieved by lower temperature treatment after incubation at 37°C for 5 hours.
A critical gas flow rate of 15 mL/min was obtained, at which the minimum detachment diameter and terminal velocity of coalesced bubble was achieved.
At Moresby, the 49th was occupied mainly digging defences and labouring; some training was achieved, although it was largely rudimentary, and a small detachment was also sent to Thursday Island.
This was achieved by limiting the remodeling reaction to the first remodeling event which we define as a single round of remodeler binding, nucleosome remodeling, and remodeler detachment from the nucleosomal DNA (Shundrovsky et al., 2006).
In drop-on-demand generation, droplet detachment can be achieved via Rayleigh Plateau instability based molten wire column break-up.
Furthermore, selective detachment of cells could also be achieved by UV light illumination on the specified gel surface.
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