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In contrast, about 50% of the cells initially adhered to the plain PLLA scaffolds were detached within the first 12 h and showed characteristics of apoptotic cell death, which was confirmed by TUNEL staining and caspase-3 activation.
Then, this trace appears to tangentially diverge from the rib tip (Additional file 2: Figure S2i) and afterwards becomes detached within the secondary xylem (Additional file 2: Figure S2c, right arrow, h and Additional file 6: Figure S6i).
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When sparsely cultured cells were treated with trypsin and EDTA, most of the mock transfectants became detached within 10 min, whereas the PPL transfectants remained attached (Fig. 3A).
The human epithelial cells grown on the surface of the hydrogels detached within 10 min with preserving cell-to-cell connection by soaking in the medium containing human saliva at 5% (v/v).
By using these reactions on ITO, cells were readily attached and then detached from ITO electrodes in a very rapid manner, with greater than 90% of the cells being detached within 5 min.
Using this approach greater than 90% of adherent cells detached within 5 min.
The biofilms started to detach within 30 min after glucose starvation as indicated by significant increase in OD of the planktonic phase after starvation, and microscopic visualization of PE confirmed that most of the biomass had dispersed after starvation.
In contrast, tablets comprising unmodified HEC detached from the mucosa within few seconds and immediately disintegrated.
By reducing culture temperature, more than 90% of the cells spontaneously detached from the dishes within several minutes without trypsin or EDTA treatment.
At 3.7 µl/cm2 of the transfection reagents, the cells detached from the plate within 24 hours.
It deployed well, and the darts detached from the animals within 2 to 24 hours.
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