Exact(6)
Using this approach greater than 90% of adherent cells detached within 5 min.
By using these reactions on ITO, cells were readily attached and then detached from ITO electrodes in a very rapid manner, with greater than 90% of the cells being detached within 5 min.
In contrast, about 50% of the cells initially adhered to the plain PLLA scaffolds were detached within the first 12 h and showed characteristics of apoptotic cell death, which was confirmed by TUNEL staining and caspase-3 activation.
The human epithelial cells grown on the surface of the hydrogels detached within 10 min with preserving cell-to-cell connection by soaking in the medium containing human saliva at 5% (v/v).
When sparsely cultured cells were treated with trypsin and EDTA, most of the mock transfectants became detached within 10 min, whereas the PPL transfectants remained attached (Fig. 3A).
Then, this trace appears to tangentially diverge from the rib tip (Additional file 2: Figure S2i) and afterwards becomes detached within the secondary xylem (Additional file 2: Figure S2c, right arrow, h and Additional file 6: Figure S6i).
Similar(52)
The biofilms started to detach within 30 min after glucose starvation as indicated by significant increase in OD of the planktonic phase after starvation, and microscopic visualization of PE confirmed that most of the biomass had dispersed after starvation.
Engineered cardiomyocyte sheets were detached spontaneously within 1 hour and floated into the aqueous media.
This was performed separately for adherent and detached leukocytes within each treatment group.
Alternatively, the probe-stained cells were detached with trypsin-EDTA, washed and fluorescence intensity was measured within 60 min by flow cytometry.
Ms. Graves said rental rates — typically for apartments within detached houses — run $1,000 a month or so for one-bedrooms, and $1,400 or $1,500 a month for two-bedrooms.
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