Sentence examples for detached using from inspiring English sources

HSCs were then washed twice with 1X PBS and detached using Accutase cell detachment solution (BD Pharmingen).

Primary human monocyte-derived Mφ were detached using a 30 min incubation with Accutase™ enzyme cell detachment medium (eBioscience).

Cells were then detached using trypsin/EDTA (0.25% w/v trypsin/0.02% EDTA of pH 7.2).

Cells were detached using 0.25% Tryspin/1 mM EDTA solution and added to corresponding tubes containing media.

Following this, cells were washed twice with cold phosphate buffer saline (PBS) and were detached using cell scrapers.

Cells were detached using trypsin-EDTA and passaged into fresh culture flasks upon reaching confluence.

The cells were then detached using trypsin, and the survival rate was assessed using MTT assays.

Target cells (5×105) were detached using non-enzymatic cell dissociation solution.

For flow cytometry, adherent cells were detached using enzyme free cell dissociation buffer (Gibco).

i3T3 cells were detached using 0.02% EDTA solution before trypsinisation of the HOKs.

Cells were detached using 0.25% trypsin/EDTA (Invitrogen, Karlsruhe, Germany) every seventh day and the cell number was determined.