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Once ESC spheres reached subconfluency, they were resuspended to detach from the plate (spheres are only loosely attached) and half of the volume was used for genomic DNA extraction of detached spheres.
The time required for sphere formation varied among the cell lines as A549, H23 and H1299 cells developed distinct large spheres after 7 days, while H125 spheres still had adherent clusters along with detached spheres.
Similar(55)
After prolonged culturing, cells detaching from spheres were predominating the culture.
As for aggressive phenotype characterisation, collagen assays showed that colosphere-forming cells were able to detach from the spheres and to migrate into the matrix as single cells.
Detached, detached.
It seems detached, wrong.
It's detached".
Each is oddly detached.
He detached mentally.
Detached, just a little.
I felt completely detached.
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