Leaves of 52-day-old plants (45 + 7 days of water stress) of the mutant and the WT grown for physiological characterization were detached (three samples per replication) and immediately fixed in liquid nitrogen.
Samples, or grains detached from the samples, were used without preparation when necessary (for OM and MRS), or prepared according to the requirement of the analytical techniques to be used.
In considering FT-IR spectra of the blue samples detached from the ceiling, we were able to identify absorption bands of ultramarine blue.
Transmission electron microscope (TEM) experiments had been performed on TNA-TiCl4 and TNA-TnB samples detached from the Ti foil and dispersed on a copper grid.
After washing with PBS, the needles were detached and the samples transferred to 1.5 ml microtubes for subsequent staining.
SECM analysis was chosen instead of conventional histological cross-sections of cells, which may be time-consuming to prepare and difficult to analyze, especially in the case of cell aggregates that can detach during sample treatment.
The observation of SiC and W18O49nanostructures separately in the TEM image indicates that the bonding between these two nanostructures might be weak and so they might have been detached during the sample preparation for TEM measurements.
After the etching processes, the tree-like silver pattern wrapping the silicon samples was detached using a NH3OH/H2O2 (3:1) solution.
The samples were detached from the FTO substrate, then dispersed in ethanol by sonication, and dropped onto a carbon film supported on a copper grid.
