In a growth chamber (25°C, 150 µmol m−2 s−1, 50% RH) leaves from well-watered plants were detached, placed abaxial-side uppermost and images acquired using an A320 infrared camera equipped with a 45° lens (FLIR Systems; http://www.flir.com).
In complementary experiments, single florets of Bd21 and Bd3-1 were detached, placed on moist filter paper in Petri dishes and inoculated with conidial suspension onto either the palea or lemma surface in order to study infection of these tissues and to identify potential differences in susceptibility between the Bd lines and between the tissues.
Three different scenarios were hypothesized: the first one considers a boulder placed on a cliff edge (joint bounded scenario JBS); in the second one, the boulder is taken up and placed below sea level (submerged scenario SMS); in the last one, the boulder is detached and placed inland (sub-aerial scenario SAS).
Oddly, though, as clubs became detached from place, ruled by offshore interests and money markets, the sport's emotional landscape was enriched.
Lateral branch roots were detached and placed over a pre-moistened cotton ball in a pre-sterilized glass vial (8 ml capacity).
To assess the ratio of water loss, fully expanded leaves from 6 individual pTRV2-SlSR1L or pTRV2-GUS-infiltrated plants were detached and placed on the bench top.
Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container.
Four days after the first inoculation, two leaves of the infected plants and a single leaf of the control plants were detached and placed on Petri dishes on moist filter paper.
At different timepoints after inoculation, primary leaves were detached and placed in a clearing solution (0.15% trichloracetic acid (w/v) in 4 1 ethanol chloroform (v/v)) for at least two days and then stored in 50% glycerol until evaluation.
At the end of the reaction, migrated cells were detached by placing transwell chambers for 15 min on ice, stained with CyQuant dye and counted using a fluorescence plate reader (M1000 Infinite®; Tecan).
