Sentence examples for detached off from inspiring English sources

Cells were gently detached off the tissue culture surface with enzyme-free cell dissociation buffer (Invitrogen) and washed once with complete medium.

293T cells were gently detached off the adherent surface with enzyme-free cell dissociation buffer (Invitrogen) and washed once with serum-free DMEM.

TL-HMECs expressing HA/FLAG-PVRL4 or HA/FLAG-GFP were gently detached off the tissue culture surface with enzyme-free cell dissociation buffer, washed, and lysed for 30 min in MCLB lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 1% NP-40) in the presence of protease and phosphatase inhibitors (Roche), followed by centrifugation at 14,000 rpm at 4°C.

The tape shows Mr Brunson (whose voice is less clear, presumably because his microphone was either detached or switched off) sympathising with Mr Major over his difficulties.

That's because the craft only has two centrally placed wheels (the two 'pogo' wheels that support the wings are detached at take off), meaning it is like trying to drop a giant bicycle out of the sky.

Nelson arrived off Newfoundland with the convoy in late May, then detached on a cruise to hunt American privateers.

Tool manufacture has also been observed among animals in the wild, for example, chimpanzees strip leaves off twigs detached from branches of trees and use the twigs for reaching termites or ants.

Some cacti produce "pads" or "joints" that can be detached or cleanly cut off.