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Moreover, the knock down of TcNPR3 in cacao leaves via cacao transient transformation resulted in decreased resistance to cacao pathogen Phytophthora capsici in detached leaf bioassays.
Therefore, we used detached leaf bioassays [ 36], a method that measures one of the major components of late leaf spot resistance as defined for cultivated peanut.
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Our leaf bioassays lasted only 5 days, which could boost survival relative to survival for longer periods required for complete larval development in the field.
As previously described, detached leaf and intact plant bioassays revealed a high correlation [ 49].
The use of detached leaf tissues in disease bioassays with Mycosphaerella banana pathogens has been reported on occasion to give inconsistent results (e.g. [ 40, 41]), with development of disease symptoms not always correlating with those typically observed on intact plants.
In the detached leaf inoculation assays, leaves were detached from at least 10 four-week-old VIGS-infiltrated plants and inoculated by dropping 5 μl of spore suspension on leaf surface.
We used a detached leaf assay, following established methods58, to assess pod borer (Helicoverpa armigera) weight gain, survival, and damage to the host plant under controlled conditions.
Desiree, a detached leaf assay was performed.
A detached leaf assay was used for semiquantitative RT-PCR.
A detached leaf method was used to evaluate the P. pachyrhizi infection [ 49].
#vbf R plants DLA: number of vector backbone free resistant plants in detached leaf assay.
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