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After incubation, cells were detached immediately with 0.5 g trypsin (Biochrom, Berlin, Germany) and 5 mmol EDTA in 1 l PBS/BSA.
Tillers containing an immature pre-meiotic spike were detached immediately after an 8-h dark period and transferred immediately to a solution of 100 mM sucrose and antisense oligodeoxynucleotides (ODNs; combination of either four sense or antisense ODNs at 10 μM each).
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Fletcher immediately detached TF11 to refuel from the oiler while TF 17 refueled from.
He immediately detached Bourlamaque and three battalions to occupy and fortify the river crossing on the portage trail about two miles (3.2 km) from the northern end of Lake George, about 6 mile from the fort.
For water loss rates measurements, leaves were detached and weighed immediately (fresh weight, FW), and were then left on the laboratory bench (humidity, 45 50%, 20 22°C) and weighed at designated time intervals (desiccated weights).
For determination of relative water content (RWC), fresh leaves of Col-0, camta1-2 and c amta1-3 were detached and weighed immediately to record FW, followed by dipping them in distilled water for 12 h.
To find out the onset, differential recovery and role of retraction in muscle atrophy and i-fat accumulation, we devised an experiment in rabbits in which the SSP tendons would be detached unilaterally, and immediately reattached, to bring about the earliest possible repair, thus precluding any muscle retraction.
Leaves were detached from plants and immediately weighed to determine their fresh weight.
For relative water content (WRA) measurements, ten 4-week-old plants with similar size in each line were detached from their roots, immediately weighed (fresh weight, FW) and then left on the laboratory bench (humidity, 45 50%, 20 22°C) until there were no further losses in weight (desiccated weight).
Detached tumour cells were immediately removed by replacement of culture medium.
The relative water content (RWC) was measured in leaves detached from plants and immediately weighed to determine their FW (fresh weight).
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