Sentence examples for detached by using from inspiring English sources

Cells of each well were detached by using 0.05% trypsin/0.02% EDTA in PBS and were counted, by dilution (1∶1) in Trypan blue, with Burker's chamber.

Cells were detached by using 3 m M EDTA in PBS.

Cells were washed with PBS and then detached by using Accutase (500 μl/well) 10 minutes at 37°C.

Cells used for quantification were detached by using trypsin-EDTA (Invitrogen) and gentle cell scraping, followed by counting on a hemocytometer (Cell-Dyn 1800, Abbott Diagnostics).

Both HEK 293T cells, mock and transduced with CLDN1 cDNA, were detached by using cell dissociation solution (Sigma-Aldrich, Saint Louis, USA) and washed with PBS.

These adherent bone marrow MSCs were detached by using trypsin-ethylenediaminetetraacetic acid (trypsin-EDTA) (0.05% wt/vol) and expanded until passage 2 prior to experimental use.

When confluent, the cells were detached by use of 0.05% trypsin-ethylenediaminetetraacetic acid (EDTA) (Invitrogen, Carlsbad, CA, USA).

For thymidine incorporation, cells in logarithmic growth phase were detached by use of 0.25% trypsin and seeded in 24-well plates in culture media with 10% FBS (1 × 10/well).

Biofilms were then detached by sonication using an ultrasound bath (Sonicor model SC-52, UK) and cultivable counts were determined on TSA plates, after aerobic incubation at 37°C.

Briefly, at the end of the incubation time for each cell treatment group, non-adherent cells were collected and combined with cells detached by trypsinization using TrypLE™ Express (Invitrogen, Carlsbad, CA) followed by centrifugation at 200 × g at 4°C. Cell pellet was then re-suspended and washed twice with cold DPBS (Invitrogen, Carlsbad).

Three days after transfection, cells were detached by accutase and used for cell viability, proliferation, migration and invasion assays, as described below.