Sentence examples for detached by two from inspiring English sources

Adherent cells were detached by two subsequent wash/centrifugation steps (0.5 % (w/v) BSA (Biochrom, Germany) and 0.1% sodium-azide (Serva, Heidelberg, Germany) in PBS) and intermittent vortexing (30s).

When the nail germinal matrix is also involved, the entire nail should be detached by making two incisions on the lateral side of the nail fold.

On my 1960s estate house (four bedrooms, detached – by 10 feet, only two resident squirrels) I pay £1,540 a year as my contribution to the civilising services of Cheshire.

Tumour lysate is collected from the soluble fraction of Neuro-2a cells detached by trypsin, after three cycles of freezing in liquid nitrogen (2 min) and thawing at 37°C (4 min) in PBS.

Briefly, UACC-62 cells (5 million) were detached by trypsinization and treated with 100 μM 2-AAPA at 37oC for 20 min.

Cells of either three ST-3 or three mock clones were detached by trypsin, counted and mixed in equal parts.

The cultured epithelium formed by the co-culture was five to six layers thick, could be detached by dispase treatment, and had sufficient strength as a sheet.

Every 48 h, cells of two independent wells were detached by trypsinisation and cell numbers were determined using a haemocytometer chamber.

To pass cells, confluent cultures were washed twice with CMF-DPBS and cells were detached by trypsin (0.025% with EDTA in CMF-DPBS) for two or three minutes at room temperature.

When the cultures reached 80% confluence, adherent cells were detached by exposure to 0.25% trypsin for five minutes, and then passaged at a dilution of 1 4.

These two modes of coupled breathers can be mapped exactly onto two solutions of a pendulum equation, detached by a separatrix mode.