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The shoots were detached as well and their length was determined.
After treatment detached as well as adherent cells were washed twice with cold PBS.
This might explain why endocytosed PIN1 in BFA-treated wild-type appears to accumulate in BFA-compartments (cell plate associated and detached) as well as the cell plate.
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And just as important: this allows it to easily detach as well.
Briefly, isolated ECFCs were detached as described above with trypsin/EDTA, and then 4×10 ECFCs were placed in the upper chambers of 24-well transwell plates with polycarbonate membranes (8-μm pores) that contained serum-free endothelial growth medium.
Yet Henderson is not quite as detached as he seems.
Stern's approach was neither as detached as Heifetz's nor as introspective as Menuhin's.
Not everybody is as detached as Robert Hargraves or as savvy as Jackie Gleason.
Infected U937 cells were rinsed and detached as described above.
This is supported because the phagocytosis of recently detached cells as well as those attached to the corneal epithelium leads to the modification of the cellular architecture facilitating the migration and destruction of deeper layers of the corneal epithelium.
Because in my own life I'm sometimes completely detached from it as well.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com