The chateau, a detached media recording studio, a detached movie theater and a swimming pool sit on more than half an acre.
But each gun casualty triggers an outburst from our sensationalist media, detached politicians and hardline gun enthusiasts, whose erotic passion for assault weapons not only borders on ammosexuality, but also makes reform grossly improbable.
Adherent Mφ were washed free of media, detached using ice cold 10 mM EDTA/PBS and cell pellets were lysed in ice-cold lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% (v/v) n-Dodecyl β-D-maltoside (Sigma), 1× protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail 2 (Sigma)).
Briefly, mitotic cells were detached into the media by gentle agitation of the plate and the remaining adherent cells were collected separately after trypsinization.
Following six hours of treatment, cells infected with a membrane-targeted GFP were detached in drug-containing media and McTNs were scored blindly.
After 10 days in culture, flasks were gently shaken to detach microglial cells to culture media.
The media are not detached observers but an integral part of the process.
Digital media has been detached from the practice of "organizing" (i.e. direct voter contact).
These media are inherently detached from the perspective of end users, leading to a disconnect between the technical analysis and the key driver.
Since August, the mass media has employed detached language and circular discussion to describe the death of Michael Brown, making the civil unrest on Monday night in Ferguson seem much more like reality television, and less like reality.
To force cells into suspension, cells were washed in PBS and exposed to a 2 mM solution of EDTA in Hank's buffer, and when fully detached, were cultured in growth media in ULC plates (Corning) for 2 hours.
