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A potentially significant second advantage of a reversibly assembling colloidal gel is the ability to passage cells without using a trypsinization process or other biochemical cell detachment method, a required step to detach cells from most substrates including current 2D and 3D matrices.
Before treatment, mono and multi-species biofilms were gently washed with PBS to detach cells not forming part of the biofilm and to remove the culture medium.
However, the enzymatic digestion process needed to detach cells from the growth surface can affect cell viability and potentially the potency and differentiation efficiency.
Samples were then agitated for three hours on a vortexer to detach cells from rock material.
A trypsin solution was used to detach cells at which point trypsin neutralizer was added and cells were then centrifuged at 300 g for 5 minutes.
This was placed in a hanging-bucket face down and then spun at designated centrifugation forces for 5 min to detach cells.
Similar(24)
Detached cells were dispersed in 4 mL of complete growth medium and gently pipette out of the well.
Detached cells from the supernatant were collected by using cytospin processing.
Detached cells, which were harvested from the supernatant, retained this rounded morphology (data not shown).
Detached cells were pelleted by centrifugation.
Detached cells were removed and the cultures were washed.
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