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RNase H+ mediated destruction of template RNA can prevent this problem and improve the sensitivity of qPCR analysis.
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As with deletion RMC, high specificity for deletion-bearing molecules is achieved through the destruction of WT template molecules by restriction endonuclease, thereby selecting for and enriching mutant molecules prior to amplification.
A plastic O-ring was mounted on the edge of the AAM template to distribute the pressure evenly and to avoid the destruction of the AAM template due to its brittle nature.
The subsequent template elimination without destruction of the mesoporous texture remains a difficult step [8].
HCV genome is an ssRNA that functions as both an mRNA and a replication template so that destruction of HCV RNA could eliminate not only virally directed protein synthesis, but also viral replication.
It set a kind of template for public mourning.
As the recently named head of the investigation into the C.I.A.'s destruction of videotapes of secret interrogations, Mr. Durham will again be the outsider trying to apply a straightforward law enforcement template to a complex set of relationships and practices.
The destruction of buildings was undeniable.
Bob Lamm tells about destruction of his house.
I ordered destruction of all chemical weapons.
Destruction of evidence.
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CEO of Professional Science Editing for Scientists @ prosciediting.com