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How then does antiestablishment destroy cohesion?
Our finding that Wapl is able to destroy cohesion long after replication is complete in cells lacking Eco1 is consistent with the latter hypothesis.
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First, Wapl should be capable of destroying cohesion long after its establishment.
In other words, it is cohesin's "releasing activity" that destroys cohesion built in the absence of Smc3 acetylation.
To understand better how Wapl destroys cohesion, we addressed the nature of its association with chromosomal cohesin.
This result demonstrates that an activity dependent on Wapl does not merely (if at all) prevent creation of cohesion; it actually destroys cohesion that has already been established.
Our experiments imply that cohesin's releasing activity not only promotes cohesin's turnover on chromosomes but also destroys cohesion once it has been established.
The simplest explanation for this result is that, in the absence of Eco1, cohesin's releasing activity destroys cohesion by disconnecting the Smc3/α-kleisin interface, permitting DNAs to escape from cohesin's embrace.
This model proposes that cohesion is the result of topologically embracing the two sister DNA molecules within a cohesin ring and that opening of the ring, due to cleavage of its Scc1 subunit by separase, liberates the sister chromatids, thereby destroying cohesion.
"This serves the motives of those who want to destroy the cohesion of the country".
A Scottie named Violet, for instance, gets an early afternoon walk while Pluto, a cocker spaniel, goes out later in the afternoon because Violet doesn't appreciate Pluto's sniffs, and walking them together would destroy the cohesion.
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