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Formally, we define the edge distance between a connected pair of reaction nodes as the inverse of the fraction of the intermediate metabolite production flux that is directed towards the destination reaction node.
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To most of my audience, the Tumblr is a destination site for reaction gifs.
The ultimate destination is the reaction center protein, where the excitation leads to charge separation that triggers transduction to chemical fuels.
Keeping track of components, we sum over the source and destination nodes for each reaction.
Previously, we used i and j to refer to the source and destination nodes in a reaction.
Clones were verified by sequencing and transferred via LR reaction into destination vector pGWB1 to obtain pPBL1 PBL1 and pPBL1 PBL1(G2A).
Clones were verified by sequencing and transferred via LR reaction into destination vector pUGW14 to obtain p35S::p35S-3xHA, p35S::BIK1-3xHandp35S::p35S(G2A)-3xHA and p35S::BIK1(G2A)-3xHA.
The aequorin-ORF was amplified from plasmid pMAQ2 by PCR using Phusion® Hot Start High-Fidelity DNA polymerase (Thermo Scientific) with primers AEQ-START/-STOP, cloned into pENTRTM/D-TOPO according to manufacturer's instructions, verified by sequencing and transferred via LR reaction into destination vector pUB-DEST to obtain pUBQ10 AEQ.
All DNA fragments were recombined via LR-reaction into destination vector pMDC123 [ 60].
In the conventional strategy for gateway cloning, the construction of two or more expression elements into tandem elements on a single plasmid requires the recombination of multiple entry clones with a destination vector in a single reaction mixture.
In the plant cell subcellular localization analysis of OsGLR2.1, we failed to shuttle OsGLR2.1 into the destination vector pGWB405 by LR reaction.
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