Sentence examples for destination expression from inspiring English sources

The coding regions were recombined into binary destination expression vectors pH7RWG2 (Cauliflower mosaic virus (CaMV) 35S promoter-driven) for C-terminal RFP (red fluorescent protein) fusions39,40 or pSITE-2CA and pSITE-2NA (2 × 35S) for N-terminal and C-terminal GFP (green fluorescent protein) fusions, respectively41.

The resultant amplicons were analyzed on 1% agarose gel and later recombined with 'Gateway Cloning Technology' entry vector pDONR201 and destination expression vector pDEST17 (Invitrogen Technologies, USA) using BP and LR reactions respectively.

Destination expression vector pDEST15 was obtained from Invitrogen.

The destination expression vector (pETG-10A, EMBL Heidelberg) contained an in-frame hexa-histidine tag upstream of the 5′ Att site.

Both shRNA and cDNA expression vectors also contained attL sites necessary for recombination with attR sites in our destination expression vectors.

The insert was transferred into pAcGFP-N1 after converting it into a destination expression vector using Gateway Conversion System (Invitrogen) by a recombination reaction using LR clonase II.

Entry vectors were set up in LR reactions to recombine the gene of interest into several destination vectors (expression vectors).

This DEST cassette allows quick transfer of any gene from a pENTR-containing entry vector (Gateway) into the DESTination-converted expression vector.

This involved direct LR recombination (Invitrogen, San Diego, CA) of the promoter containing shRNA/cDNA entry vector to any one of promortless lentiviral destination vectors with expression of different markers or drug selection genes.

To generate a series of pFLIPi destination vectors for expression in E. coli with different fluorescent protein combinations, the 5′ fluorescent protein genes were exchanged using the XhoI and KpnI restriction sites and the C-terminal fluorescent protein genes were exchanged using the SpeI and PspOMI sites (Table 1; Supplementary file 1).

Via Gateway LR Clonase Mix (Invitrogen) the PpAOS1-YFP and PpAOS2-YFP coding sequence was transferred into a modified pCAMBIA33.1 plasmid, a plant expression destination vector containing the cauliflower mosaic virus (CaMV) 35S-promoter, an attR1/R2 gateway cassette and a CaMV- 35S-terminator [ 52].