We also used the R163STOP protein in both these amorphous aggregation assays to assess its relative chaperone ability compared to the wild-type protein, but due to its destabilized structure only a limited number of assays could be performed.
Nuclear dsRNA-binding proteins are known to destabilize structured pre-mRNA (Danin-Kreiselman et al, 2003; Kadener et al, 2009).
The Dictyostelium coronin CorA is involved in destabilizing actin structures [ 21, 22].
The SWI/SNF family is one of the most-studied families of ATP-dependent chromatin remodeling complexes, which regulate gene expression by destabilizing nucleosome structures to alter the DNA accessibility for transcription factors (Cairns, 2007; Hargreaves and Crabtree, 2011).
Analysis of the three-dimensional structure showed that substitution of Phe 151 with Leu produces a hydrophobic cavity in the GSTP1 core, thereby destabilizing its structure.
Because protein structures are the result of their amino acid sequences, some changes can dramatically change the properties of a protein by destabilizing the structure or changing the surface of the protein in a way that changes its interaction with other proteins and molecules.
Contacts involving many beta-branched hydrophobic residues in the crowded protein core may generate strain in helices, destabilizing the structure.
Acetylation, which neutralizes the positive charge of the lysine side-chain, is thought to weaken histone-DNA or nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and allowing greater accessibility [10].
Furthermore, destabilizing 5' mRNA structure need not require significant synonymous substitutions to rare codons.
Low levels of proBias enabled fissures to reach toward the SMS center, destabilizing the structure and causing chaotic, uncontrolled growth.
