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In this step, MCAK-mediated microtubule destabilization is required to remove laterally attached microtubules.
While protein destabilization is required for hydrogel formation, too much denaturation becomes a hindrance to protein functionality and biocompatibility.
We show that stabilization of axonal microtubules significantly reduced caspase-3 activation within the axon suggesting that microtubule destabilization is required for caspase activation.
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More dramatic destabilizations were required to obtain data at pH 7.
However, the system relies on the constant application of the ligand Shld1, which binds to the fused destabilization domain, until protein degradation is required and as this is unlikely to be possible during mosquito stages, the method cannot yet be used for studying the consequences of protein depletion during either the mosquito or liver stages.
These results suggest that a Nutlin-3 sensitive domain of Hdm2 is required for Ku70 destabilization.
These observations suggest that, similar to protein degradation, CaN activation in the amygdala is required for the destabilization and enhancement of IA memory, while CaN activation in the hippocampus and mPFC is required for the enhancement of IA memory.
Furthermore, a micro-infusion of β-lac with ANI into the amygdala prevented the disruption of IA memory by protein synthesis inhibition, suggesting that protein degradation in the amygdala is required for the destabilization of reactivated IA memory.
Instead, our data are consistent with the notion that Wnt11r-mediated destabilization of the pre-pouch epithelium is required for cells to be guided by local sources of Fgf8a in the ventral nkx2.5-positive mesoderm (Fig. 6Q).
Hdm2 mutant lacking ubiquitin ligase activity (C464S mutant) 37 did not influence Ku70 levels (Fig. 2a) suggesting that the ubiquitin ligase activity of Hdm2 is required for Ku70 destabilization.
Filament formation requires ATP binding, but ATP hydrolysis is required only for filament destabilization and turnover [19], [24].
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CEO of Professional Science Editing for Scientists @ prosciediting.com